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rig i reporter cell line  (InvivoGen)


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    InvivoGen rig i reporter cell line
    Rig I Reporter Cell Line, supplied by InvivoGen, used in various techniques. Bioz Stars score: 94/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rig+i+reporter+cell+line+hek+lucia+rig/HEK-Lucia+RIG-I+Cells/pm41603157-242-1-7
    Average 94 stars, based on 18 article reviews
    rig i reporter cell line - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    other:

    Article Title: A Cell-Based Screen Identifies HDAC Inhibitors as Activators of RIG-I Signaling
    Article Snippet: RIG-I reporter cell line HEK-LuciaTM RIG-I (Invivogen, #hkl-hrigi) was cultured in Dulbecco’s Modified Eagles Medium (DMEM), High Glucose (HyCloneTM) supplemented with 10% Fetal Bovine Serum (FBS) (Bio-Techne R&D Systems #S11550H), 30 μg/ml blasticidin, 100 μg/ml.

    Article Title: A Cell-Based Screen Identifies HDAC Inhibitors as Activators of RIG-I Signaling.
    Article Snippet: RIG-I reporter cell line HEK-LuciaTM RIG-I (Invivogen, #hklhrigi) was cultured in Dulbecco’s Modified Eagles Medium (DMEM), High Glucose (HyCloneTM) supplemented with 10% Fetal Bovine Serum (FBS) (Bio-Techne R&D Systems #S11550H), 30 μg/ml blasticidin, 100 μg/ml.

    Article Title: A cell-based screen identifies HDAC inhibitors and PLK inhibitor as activators of RIG-I signaling
    Article Snippet: RIG-I reporter cell line HEK-Lucia TM RIG-I (Invivogen, #hkl-hrigi) was cultured in Dulbecco’s Modified Eagles Medium (DMEM), High Glucose (HyClone TM ) supplemented with 10% Fetal Bovine Serum (FBS) (Bio-Techne R&D Systems #S11550H), 30 μg/ml blasticidin, 100 μg/ml Normocin TM and 100 μg/ml of Zeocin TM .

    Control:

    Article Title: Template-Directed RIG-I Agonist Assembly for Targeted Cancer Immunotherapy
    Article Snippet: A 19-mer 5’ppp-dsRNA positive control for RIG-I activation was obtained from InvivoGen (San Diego, CA; Catalog No. tlrl-3prna). .. The RIG-I reporter cell line HEK-LuciaTM RIG-I cells (Catalog Code, hkl-hrigi, InvivoGen) and the control cell line HEK-LuciaTM Null cells (Catalog No. hkl-null, InvivoGen), and Mouse skin melanoma cells (B16-F10) were cultured in Dulbecco’s Modified Eagles Medium (DMEM, Gibco). .. All culture media contained 10% fetal bovine serum (serum, Gibco), 100 U/mL of penicillin, and 100 mg/mL of streptomycin.

    Cell Culture:

    Article Title: Template-Directed RIG-I Agonist Assembly for Targeted Cancer Immunotherapy
    Article Snippet: A 19-mer 5’ppp-dsRNA positive control for RIG-I activation was obtained from InvivoGen (San Diego, CA; Catalog No. tlrl-3prna). .. The RIG-I reporter cell line HEK-LuciaTM RIG-I cells (Catalog Code, hkl-hrigi, InvivoGen) and the control cell line HEK-LuciaTM Null cells (Catalog No. hkl-null, InvivoGen), and Mouse skin melanoma cells (B16-F10) were cultured in Dulbecco’s Modified Eagles Medium (DMEM, Gibco). .. All culture media contained 10% fetal bovine serum (serum, Gibco), 100 U/mL of penicillin, and 100 mg/mL of streptomycin.

    Modification:

    Article Title: Template-Directed RIG-I Agonist Assembly for Targeted Cancer Immunotherapy
    Article Snippet: A 19-mer 5’ppp-dsRNA positive control for RIG-I activation was obtained from InvivoGen (San Diego, CA; Catalog No. tlrl-3prna). .. The RIG-I reporter cell line HEK-LuciaTM RIG-I cells (Catalog Code, hkl-hrigi, InvivoGen) and the control cell line HEK-LuciaTM Null cells (Catalog No. hkl-null, InvivoGen), and Mouse skin melanoma cells (B16-F10) were cultured in Dulbecco’s Modified Eagles Medium (DMEM, Gibco). .. All culture media contained 10% fetal bovine serum (serum, Gibco), 100 U/mL of penicillin, and 100 mg/mL of streptomycin.

    Luciferase:

    Article Title: Non-Targeting shRNA-Encoded Plasmid DNA Enhances Protective Immunity Through RIDD-RIG-I Signaling Pathway in the Zika Virus Animal Model.
    Article Snippet: Human embryonic kidney (HEK) 293T cells (ATCC, CRL-3216) were used for siRNA transfection to assess protein expression of RIDD-related genes. .. The RIG-I reporter cell line, HEK-Lucia RIG-I (InvivoGen; Cat. # hkl-hrigi), was used in luciferase activity assays following siRNA transfection to evaluate RIG-I pathway activation. .. The TLR3-IFN- β-Luc reporter cell line, derived from HEK293 cells (provided by Dr. Tsung-Hsien Chuang, NHRI, Taiwan) [ 29 ], was generated by stable transfection with a TLR3 expression vector and an IFN- β promoter-driven MetLuc reporter construct (Clontech Laboratories, Inc.).

    Activity Assay:

    Article Title: Non-Targeting shRNA-Encoded Plasmid DNA Enhances Protective Immunity Through RIDD-RIG-I Signaling Pathway in the Zika Virus Animal Model.
    Article Snippet: Human embryonic kidney (HEK) 293T cells (ATCC, CRL-3216) were used for siRNA transfection to assess protein expression of RIDD-related genes. .. The RIG-I reporter cell line, HEK-Lucia RIG-I (InvivoGen; Cat. # hkl-hrigi), was used in luciferase activity assays following siRNA transfection to evaluate RIG-I pathway activation. .. The TLR3-IFN- β-Luc reporter cell line, derived from HEK293 cells (provided by Dr. Tsung-Hsien Chuang, NHRI, Taiwan) [ 29 ], was generated by stable transfection with a TLR3 expression vector and an IFN- β promoter-driven MetLuc reporter construct (Clontech Laboratories, Inc.).

    Transfection:

    Article Title: Non-Targeting shRNA-Encoded Plasmid DNA Enhances Protective Immunity Through RIDD-RIG-I Signaling Pathway in the Zika Virus Animal Model.
    Article Snippet: Human embryonic kidney (HEK) 293T cells (ATCC, CRL-3216) were used for siRNA transfection to assess protein expression of RIDD-related genes. .. The RIG-I reporter cell line, HEK-Lucia RIG-I (InvivoGen; Cat. # hkl-hrigi), was used in luciferase activity assays following siRNA transfection to evaluate RIG-I pathway activation. .. The TLR3-IFN- β-Luc reporter cell line, derived from HEK293 cells (provided by Dr. Tsung-Hsien Chuang, NHRI, Taiwan) [ 29 ], was generated by stable transfection with a TLR3 expression vector and an IFN- β promoter-driven MetLuc reporter construct (Clontech Laboratories, Inc.).

    Activation Assay:

    Article Title: Non-Targeting shRNA-Encoded Plasmid DNA Enhances Protective Immunity Through RIDD-RIG-I Signaling Pathway in the Zika Virus Animal Model.
    Article Snippet: Human embryonic kidney (HEK) 293T cells (ATCC, CRL-3216) were used for siRNA transfection to assess protein expression of RIDD-related genes. .. The RIG-I reporter cell line, HEK-Lucia RIG-I (InvivoGen; Cat. # hkl-hrigi), was used in luciferase activity assays following siRNA transfection to evaluate RIG-I pathway activation. .. The TLR3-IFN- β-Luc reporter cell line, derived from HEK293 cells (provided by Dr. Tsung-Hsien Chuang, NHRI, Taiwan) [ 29 ], was generated by stable transfection with a TLR3 expression vector and an IFN- β promoter-driven MetLuc reporter construct (Clontech Laboratories, Inc.).



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